Application
Confocal immunofluorescent analysis of CBS Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis in Raji cell line, rat brain and cerebellum tissue lysates (35ug/lane).Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.Application
Immunohistochemical analysis of paraffin-embedded H. brain section using CBS Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | CBS Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cystathionine beta-synthase, Beta-thionase, Serine sulfhydrase, CBS |
| Product description | CBS acts as a homotetramer to catalyze the conversion of homocysteine to cystathionine, the first step in the transsulfuration pathway. This protein is allosterically activated by adenosyl-methionine and uses pyridoxal phosphate as a cofactor. Defects in this gene can cause cystathionine beta-synthase deficiency (CBSD), which can lead to homocystinuria.1) Ravel,C., et.al., PLoS ONE(8), E6540 (2009) |
| Immunogen | This CBS antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 301-330 amino acids from the Central region of human CBS. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CBS |
|---|---|
| Alternative Names | Cystathionine beta-synthase Beta-thionase Serine sulfhydrase CBS |
| Molecular Weight(MW) | 61 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Confocal immunofluorescent analysis of CBS Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CBS Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis in Raji cell line, rat brain and cerebellum tissue lysates (35ug/lane).
Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Immunohistochemical analysis of paraffin-embedded H. brain section using CBS Antibody . Antibody was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:25 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
