Application
Immunohistochemistry of CCDC134 in human brain tissue with CCDC134 antibody at 2.5 μg/mL.Application
Western blot analysis of CCDC134 in rat brain tissue lysate with CCDC134 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of CDCC134 in human brain tissue with CDCC134 antibody at 20 μg/mL.| Product Name | CCDC134 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCDC134 Antibody: dJ821D11.3, Coiled-coil domain-containing protein 134 |
| Product description | CCDC134 Antibody: The coiled-coil domain is a common protein motif that is often involved in protein oligomerization and is found in proteins such as transcription factors and intermediate filaments. One such protein is CCDC134, a recently identified secretory protein that has been found to inhibit the transcriptional activity of the Elk1 protein. Overexpression CCDC134 also inhibited the phosphorylation of Erk and JNK/SAPK but not p38 MAPK, while specific siRNA against CCDC134 activated Elk1 transcriptional activity and the phosphorylation of Erk and JNK/SAPK, suggesting a potential inhibiting role of CCDC134 in MAPK-mediated Elk1 transcription. CCDC134 is widely expressing in normal adult tissues, tumors, and cell lines.1) Steinmetz MO, Jelesarov I, Matousek WM, et al. Molecular basis of coiled-coil formation. Proc. Natl. Acad. Sci. USA2007; 104:7062-7. |
| Immunogen | CCDC134 antibody was raised against a 17 amino acid synthetic peptide near the amino terminus of human CCDC134. The immunogen is located within the first 50 amino acids of CCDC134. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CCDC134 antibody can be used for detection of CCDC134 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCDC134 |
|---|---|
| Alternative Names | CCDC134 Antibody: dJ821D11.3 Coiled-coil domain-containing protein 134 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Immunohistochemistry of CCDC134 in human brain tissue with CCDC134 antibody at 2.5 μg/mL.
Application
Western blot analysis of CCDC134 in rat brain tissue lysate with CCDC134 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of CDCC134 in human brain tissue with CDCC134 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1463 - Rat Brain Tissue Lysate |
|---|---|
| Application Notes | CCDC134 antibody can be used for detection of CCDC134 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCDC134 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCDC134 Antibody is supplied in PBS containing 0.02% sodium azide. |
