Application
Immunofluorescence of CCDC141 in mouse brain tissue with CCDC141 antibody at 20 μg/mL.Application
Immunohistochemistry of CCDC141 in mouse brain tissue with CCDC141 antibody at 5 μg/mL.Application
Western blot analysis of CCDC141 in SK-N-SH cell tissue lysate with CCDC141 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Product Name | CCDC141 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCDC141 Antibody: CAMDI, Coiled-coil domain-containing protein 141 |
| Product description | CCDC141 Antibody: Coiled-coil domain-containing protein 141 (CCDC141), also known as CAMDI, is a recently identified disrupted in schizophrenia 1 (DISC1)-binding protein that also associates with myosin II. CCDC141 preferentially associates with phosphomyosin II and causes an accumulation of phosphomyosin II at the centrosome in a DISC1-dependent manner. Knockdown of CCDC141 expression by RNAi led to severely impaired radial migration with disoriented chromosomes, suggesting that CCDC141 is required for radial migration through DISC1 and myosin II-mediated centrosome positioning during neuronal development.1) Fukuda T, Sugita S, Inatome R, et al. CAMDI, a novel disrupted in schizophrenia 1 (DISC1)-binding protein, is required for radial migration. J. Biol. Chem. 2010; 285:40554-61. |
| Immunogen | CCDC141 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CCDC141. The immunogen is located within amino acids 40 - 90 of CCDC141. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CCDC141 antibody can be used for detection of CCDC141 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCDC141 |
|---|---|
| Alternative Names | CCDC141 Antibody: CAMDI Coiled-coil domain-containing protein 141 |
| Tissue Specificity | At least four isoforms of CCDC141 are known to exist. |
Application
Immunofluorescence of CCDC141 in mouse brain tissue with CCDC141 antibody at 20 μg/mL.
Application
Immunohistochemistry of CCDC141 in mouse brain tissue with CCDC141 antibody at 5 μg/mL.
Application
Western blot analysis of CCDC141 in SK-N-SH cell tissue lysate with CCDC141 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | CCDC141 antibody can be used for detection of CCDC141 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCDC141 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCDC141 Antibody is supplied in PBS containing 0.02% sodium azide. |
