Application
Immunofluorescence of CCDC22 in human brain tissue with CCDC22 antibody at 20 μg/mL.Application
Western blot analysis of CCDC22 in 293 cell lysate with CCDC22 antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CCDC22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCDC22 Antibody: JM1, CXorf37, JM1, Coiled-coil domain-containing protein 22 |
| Product description | CCDC22 Antibody: CCDC22 is a recently identified coiled-coil domain-containing protein that has been shown to bind copines, which are calcium-dependent, membrane-binding proteins that may function in calcium signaling. In rat, CCDC22 has been observed to localize in multiple regions of the brain, including the prefrontal and somatosensory cortex, dentate gyrus and thalamus, and in the ipsilateral motor neurons of the spinal cord after sciatic nerve transection suggesting that it may play a role in neuronal injury response. The human CCDC22 gene has been identified as a novel candidate gene for syndromic X-linked intellectual disability (XLID).1) Tomsig JL, Snyder SL, and Creutz CE. Identification of targets for calcium signaling through the copine family of proteins. J. Biol. Chem. 2003; 278:10048-54. |
| Immunogen | CCDC22 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CCDC22. The immunogen is located within amino acids 530 - 580 of CCDC22. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFWB |
| CCDC22 antibody can be used for detection of CCDC22 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCDC22 |
|---|---|
| Alternative Names | CCDC22 Antibody: JM1 CXorf37 JM1 Coiled-coil domain-containing protein 22 |
| Sequence Similarities | 2) Cat. No. 10-301 - Human Brain Tissue Slide |
| Tissue Specificity | At least three isoforms of CCDC22 are known to exist; this antibody will detect the two largest isoforms. |
Application
Immunofluorescence of CCDC22 in human brain tissue with CCDC22 antibody at 20 μg/mL.
Application
Western blot analysis of CCDC22 in 293 cell lysate with CCDC22 antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - 293 Cell Lysate |
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| Application Notes | CCDC22 antibody can be used for detection of CCDC22 by Western blot at 1 - 2 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCDC22 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCDC22 Antibody is supplied in PBS containing 0.02% sodium azide. |
