Application
Western blot analysis of CCDC98 in human breast tissue lysate in (A) the absence and (B) presence of blocking peptide with CCDC98 antibody at 1 μg/mL.Application
Immunohistochemistry of CCDC98 in human breast tissue with CCDC98 antibody at 5 μg/mL.| Product Name | CCDC98 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCDC98 Antibody: ABRA1, CCDC98, ABRA1, UNQ496/PRO1013, BRCA1-A complex subunit Abraxas, Coiled-coil domain-containing protein 98 |
| Product description | CCDC98 Antibody: CCDC98, also known as Abraxas 1, was identified through protein binding studies using the breast and ovarian predisposition protein BRCA1 as the binding target. CCDC98 recruits RAP80, a ubiquitin-binding protein, to BRCA1, allowing the formation of BRCA1 foci in response to DNA damage caused by ionizing radiation. Both CCDC98 and RAP80 are required for DNA damage resistance, G2-M checkpoint control, and DNA repair. Cells depleted of either CCDC98 or RAP80 exhibited increased sensitivity to ionizing radiation, although not as much as in BRCA1-depleted cells, suggesting that CCDC98 and RAP80 control only part of the DNA damage response role of BRCA1. At least two isoforms of CCDC98 are known to exist.1) Wang B, Matsuoka S, Balliff BA, et al. Abraxas and RAP80 form a BRCA1 protein complex required for the DNA damage response. Science2007; 316:1194-1198. |
| Immunogen | CCDC98 antibody was raised against a 15 amino acid synthetic peptide from near the amino terminus of human CCDC98. The immunogen is located within amino acids 80 - 130 of CCDC98. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| CCDC98 antibody can be used for detection of CCDC98 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | FAM175A |
|---|---|
| Alternative Names | CCDC98 Antibody: ABRA1 CCDC98 ABRA1 UNQ496/PRO1013 BRCA1-A complex subunit Abraxas Coiled-coil domain-containing protein 98 |
| Sequence Similarities | 2) Cat. No. 10-001 - Human Breast Tissue Slide |
Application
Western blot analysis of CCDC98 in human breast tissue lysate in (A) the absence and (B) presence of blocking peptide with CCDC98 antibody at 1 μg/mL.
Application
Immunohistochemistry of CCDC98 in human breast tissue with CCDC98 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1311 - Human Breast Tissue Lysate |
|---|---|
| Application Notes | CCDC98 antibody can be used for detection of CCDC98 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCDC98 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCDC98 Antibody is supplied in PBS containing 0.02% sodium azide. |
