Application
CCDC99 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Product Name | CCDC99 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Protein Spindly {ECO:0000255|HAMAP-Rule:MF_03041}, hSpindly, Arsenite-related gene 1 protein, Coiled-coil domain-containing protein 99 {ECO:0000255|HAMAP-Rule:MF_03041}, Rhabdomyosarcoma antigen MU-RMS-404A, Spindle apparatus coiled-coil domain-containing |
| Product description | CCDC99 is required for the localization of dynein and dynactin to the mitotic kintochore. Dynein is believed to control the initial lateral interaction between the kinetochore and spindle microtubules and to facilitate the subsequent formation of end-on kinetochore-microtubule attachments mediated by the NDC80 complex. Also required for correct spindle orientation. Does not appear to be required for the removal of spindle assembly checkpoint (SAC) proteins from the kinetochore upon bipolar spindle attachment.1) Barisic, M., et al. Mol. Biol. Cell 21(12):1968-1981(2010) |
| Immunogen | This CCDC99 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 459-488 amino acids from the C-terminal region of human CCDC99. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | SPDL1 |
|---|---|
| Alternative Names | Protein Spindly {ECO:0000255|HAMAP-Rule:MF_03041} hSpindly Arsenite-related gene 1 protein Coiled-coil domain-containing protein 99 {ECO:0000255|HAMAP-Rule:MF_03041} Rhabdomyosarcoma antigen MU-RMS-404A Spindle apparatus coiled-coil domain-containing |
| Molecular Weight(MW) | 70 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
CCDC99 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in A549 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
