Application
Confocal immunofluorescent analysis of CCHCR1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) .DAPI was used to stain the cell nuclear (blue).Application
Flow cytometric analysis of widr cells using CCHCR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CCHCR1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CCHCR1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Coiled-coil alpha-helical rod protein 1, Alpha-helical coiled-coil rod protein, Putative gene 8 protein, Pg8, CCHCR1, C6orf18, HCR |
| Product description | CCHCR1 may be a regulator of keratinocyte proliferation or differentiation.1) Asumalahti,K., et.al., Hum. Mol. Genet.(10), 1533-1542 (2000) |
| Immunogen | This CCHCR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 599-627 amino acids from the Central region of human CCHCR1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCHCR1 |
|---|---|
| Alternative Names | Coiled-coil alpha-helical rod protein 1 Alpha-helical coiled-coil rod protein Putative gene 8 protein Pg8 CCHCR1 C6orf18 HCR |
| Molecular Weight(MW) | 89 kDa |
Application
Confocal immunofluorescent analysis of CCHCR1 Antibody with 293 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) .DAPI was used to stain the cell nuclear (blue).
Application
Flow cytometric analysis of widr cells using CCHCR1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with CCHCR1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
