Application
PTHLH antibody [N1C3] detects PTHLH protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. PTHLH antibody [N1C3] diluted at 1:500.Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE diluted at 1:1000Application
PTHLH antibody [N1C3] detects PTHLH protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTHLH protein stained by PTHLH antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Product Name | PTHLH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to PTHLH (parathyroid hormone-like hormone)The protein encoded by this gene is a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Four alternatively spliced transcript variants encoding two distinct isoforms have been observed. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, in-frame and downstream of the initiator AUG codon, to give rise to nuclear forms of this hormone. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human PTHLH. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Dog, Pig, Rabbit, Rhesus Monkey, BovineMouse (85%), Rat (89%), Dog (92%), Pig (91%), Rabbit (88%), Rhesus Monkey (99%), Bovine (91%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BDE2 antibody HHM antibody MGC14611 antibody PLP antibody PTHR antibody PTHRP antibody PTHLH antibody osteostatin antibody parathyroid hormone-related protein antibody PTH-related protein antibody parathyroid hormone-like related protein antibody |
|---|---|
| Molecular Weight(MW) | 20 kDa (note) (2) |
| Cellular Localization | Cytoplasm , Nucleus , Secreted |
Application
PTHLH antibody [N1C3] detects PTHLH protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung carcinoma. PTHLH antibody [N1C3] diluted at 1:500.
Application
Sample (30 ug of whole cell lysate) A: HepG2 B: HCT116 12% SDS PAGE diluted at 1:1000
Application
PTHLH antibody [N1C3] detects PTHLH protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: HepG2 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: PTHLH protein stained by PTHLH antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 μm.| Positive Control | A549 , HepG2 , HCT116 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
