Application
Antibody used in IP on Human NT2 at: 1:50.Application
Antibody used in WB on human NT2 line at 1:500.Application
Antibody used in IP on Mouse Brain at: 1:50.Application
Antibody used in WB on Human Jurkat 0.125 ug/ml.| Product Name | CCNB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCNB1, CCNB |
| Product description | CCNB1 is a regulatory protein involved in mitosis. CCNB1 complexes with p34 (cdc2) to form the maturation-promoting factor (MPF).The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites.1) Zhao, M., (2006) Exp Oncol 28 (1), 44-48. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCNB1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CCNB1 antibody can be used for detection of CCNB1 by ELISA at 1:312500. CCNB1 antibody can be used for detection of CCNB1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNB1 |
|---|---|
| Alternative Names | CCNB1 CCNB |
| Molecular Weight(MW) | 48 kDa |
Application
Antibody used in IP on Human NT2 at: 1:50.
Application
Antibody used in WB on human NT2 line at 1:500.
Application
Antibody used in IP on Mouse Brain at: 1:50.
Application
Antibody used in WB on Human Jurkat 0.125 ug/ml.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CCNB1 antibody can be used for detection of CCNB1 by ELISA at 1:312500. CCNB1 antibody can be used for detection of CCNB1 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CCNB1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
