Application
CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Product Name | CCNB1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | G2/mitotic-specific cyclin-B1, CCNB1, CCNB |
| Product description | The protein encoded by this gene is a regulatory protein involved in mitosis. The gene product complexes with p34(cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript, that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. [provided by RefSeq].1) Kreis, N.N., et al. Oncogene 29(41):5591-5603(2010) |
| Immunogen | This CCNB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 241-268 amino acids from the Central region of human CCNB1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CCNB1 |
|---|---|
| Alternative Names | G2/mitotic-specific cyclin-B1 CCNB1 CCNB |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
CCNB1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human uterus tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in MDA-MB435 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
