Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CCNB1IP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CCNB1IP1 Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | CCNB1IP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | E3 ubiquitin-protein ligase CCNB1IP1, 632-, Cyclin-B1-interacting protein 1, Human enhancer of invasion 10, CCNB1IP1, C14orf18, HEI10 |
| Product description | HEI10 is a member of the E3 ubiquitin ligase family and functions in progression of the cell cycle through G(2)/M.1) Sugiyama, N., et al. Mol. Cell Proteomics 6(6):1103-1109(2007) |
| Immunogen | This CCNB1IP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 199-228 amino acids from the C-terminal region of human CCNB1IP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNB1IP1 |
|---|---|
| Alternative Names | E3 ubiquitin-protein ligase CCNB1IP1 632- Cyclin-B1-interacting protein 1 Human enhancer of invasion 10 CCNB1IP1 C14orf18 HEI10 |
| Molecular Weight(MW) | 32 kDa |
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with CCNB1IP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Flow cytometry analysis of NCI-H460 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CCNB1IP1 Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
