Application
Western blot analysis in CEM cell line lysates (35ug/lane).Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).| Product Name | CCNF Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyclin-F, F-box only protein 1, CCNF, FBX1, FBXO1 |
| Product description | This gene encodes a member of the cyclin family. Cyclins are important regulators of cell cycle transitions through their ability to bind and activate cyclin-dependent protein kinases. This member also belongs to the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class and it was one of the first proteins in which the F-box motif was identified.1) D'Angiolella, V., et al. Nature 466(7302):138-142(2010) |
| Immunogen | This CCNF antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 496-524 amino acids from the Central region of human CCNF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCNF |
|---|---|
| Alternative Names | Cyclin-F F-box only protein 1 CCNF FBX1 FBXO1 |
| Molecular Weight(MW) | 88 kDa |
Application
Western blot analysis in CEM cell line lysates (35ug/lane).
Application
Western blot analysis in mouse liver tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
