Application
Immunohistochemistry of CCP110 in rat colon tissue with CCP110 antibody at 5 μg/mL.Application
Western blot analysis of CCP110 in human colon tissue lysate with CCP110 antibody at 1 μg/mL.Application
Immunofluorescence of CCP110 in rat colon tissue with CCP110 antibody at 20 μg/mL.| Product Name | CCP110 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCP110 Antibody: CP110, Cep110, CEP110, CP110, KIAA0419, Centriolar coiled-coil protein of 110 kDa, Centrosomal protein of 110 kDa |
| Product description | CCP110 Antibody: CCP110 is a centrosomal protein required for the centrosome to function as a microtubule organizing center and plays an essential role in centrosome duplication. It is a target of cyclin-dependent kinases (CDKs); disruption of CCP110 phosphorylation leads to unscheduled centrosome separation and polyploidy. CCP110 is also a target of SCF (Cyclin F) ubiquitin ligase complex, leading to its degradation, suggesting that this complex controls centrosome homeostasis and mitotic fidelity through CCP110 degradation. The centriolar kinesin Kif24 also interacts with CP110 and remodel microtubules and regulate ciliogenesis.1) Chen Z, Indjeian VB, McManus M, et al. CP110, a cell cycle-dependent CDK substrate, regulates centrosome duplication in human cells. Dev. Cell 2002; 3:339-50. |
| Immunogen | CCP110 antibody was raised against a 17 amino acid synthetic peptide near the carboxy terminus of human CCP110. The immunogen is located within the last 50 amino acids of CCP110. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CCP110 antibody can be used for detection of CCP110 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCP110 |
|---|---|
| Alternative Names | CCP110 Antibody: CP110 Cep110 CEP110 CP110 KIAA0419 Centriolar coiled-coil protein of 110 kDa Centrosomal protein of 110 kDa |
| Molecular Weight(MW) | Predicted: 110 kDa Observed: 110 kDa |
| Tissue Specificity | Two isoforms of CCP110 are known to exist; this antibody will only detect the longer isoform |
| Entrez Gene | 9738 |
|---|---|
| Protein Accession | NP_001185951 |
Application
Immunohistochemistry of CCP110 in rat colon tissue with CCP110 antibody at 5 μg/mL.
Application
Western blot analysis of CCP110 in human colon tissue lysate with CCP110 antibody at 1 μg/mL.
Application
Immunofluorescence of CCP110 in rat colon tissue with CCP110 antibody at 20 μg/mL.| Positive Control | 1) Cat. No. 1320 - Human Colon Tissue Lysate |
|---|---|
| Application Notes | CCP110 antibody can be used for detection of CCP110 by Western blot at 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCP110 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCP110 Antibody is supplied in PBS containing 0.02% sodium azide. |
