CCPG1 acts as an assembly platform for Rho protein signaling complexes. It limits guanine nucleotide exchange activity of MCF2L toward RHOA, which results in an inhibition of both its transcriptional activation ability and its transforming activity. It does not inhibit activity of MCF2L toward CDC42, or activity of MCF2 toward either RHOA or CDC42. It may be involved in cell cycle regulation.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CCPG1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
WB
CCPG1 antibody can be used for detection of CCPG1 by ELISA at 1:312500. CCPG1 antibody can be used for detection of CCPG1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human HepG2 at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1211 - HepG2 Cell Lysate
Application Notes
CCPG1 antibody can be used for detection of CCPG1 by ELISA at 1:312500. CCPG1 antibody can be used for detection of CCPG1 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CCPG1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.