Application
Immunofluorescence of CCR3 in Human Spleen tissue with CCR3 antibody at 20 μg/mL.Application
Immunohistochemistry of CCR3 in human spleen tissue with CCR3 antibody at 10 μg/mL.Application
Western blot analysis of CCR3 in human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 μg/mL (lane 2), and 2 μg/mL in the presence of blocking peptide (lane 3).| Product Name | CCR3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CCR3 Antibody: CKR3, CD193, CMKBR3, CC-CKR-3, C-C chemokine receptor type 3, Eosinophil eotaxin receptor, C-C CKR-3 |
| Product description | CCR3 Antibody: Human immunodeficiency virus (HIV) and related virus require coreceptors to infect target cells. Some G protein-coupled receptors including CCR5, CXCR4, CCR3, CCR2b, CCR8, GPR15, STRL33, and CX3CR1 in the chemokine receptor family were recently identified as HIV coreceptors. CCR5, CXCR4 and CCR3 are the principal receptors for HIV fusion and entry of target cells. CCR3 facilitates infection by a subset of virus. CCR3 and CCR5 promote efficient infection of microglia, the major target cells in the CNS. High levels of CCR3 and CXCR4 expression were found on the neurons from both the central and peripheral nervous systems. The CCR3 ligand, eotaxin, and an anti-CCR3 antibody inhibited HIV infection of microglia. These results indicate CCR3 plays an important role in HIV infection of CNS.1) Feng Y, Broder CC, Kennedy PE, et al. HIV-1 entry cofactor: functional cDNA cloning of a seven-transmembrane, G protein-coupled receptor. Science 1996; 272:872-7. |
| Immunogen | CCR3 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human CCR3. The immunogen is located within the last 50 amino acids of CCR3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCR3 |
|---|---|
| Alternative Names | CCR3 Antibody: CKR3 CD193 CMKBR3 CC-CKR-3 C-C chemokine receptor type 3 Eosinophil eotaxin receptor C-C CKR-3 |
| Molecular Weight(MW) | Predicted: 39, 41 kDa Observed: 52 kDa |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
| Tissue Specificity | At least three isoforms of CCR3 are known to exist; this antibody will detect all three isoforms. |
Application
Immunofluorescence of CCR3 in Human Spleen tissue with CCR3 antibody at 20 μg/mL.
Application
Immunohistochemistry of CCR3 in human spleen tissue with CCR3 antibody at 10 μg/mL.
Application
Western blot analysis of CCR3 in human spleen tissue lysates with CCR3 antibody at 1 (lane 1) and 2 μg/mL (lane 2), and 2 μg/mL in the presence of blocking peptide (lane 3).| Positive Control | 1) Cat. No. 1306 - Human Spleen Tissue Lysate |
|---|---|
| Application Notes | CCR3 antibody can be used for the detection of CCR3 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CCR3 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CCR3 Antibody is supplied in PBS containing 0.02% sodium azide. |
