Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.Application
CCT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Product Name | CCT8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | T-complex protein 1 subunit theta, TCP-1-theta, CCT-theta, Renal carcinoma antigen NY-REN-15, CCT8, C21orf112, CCTQ, KIAA0002 |
| Product description | Molecular chaperone; CCT8 assists the folding of proteins upon ATP hydrolysis. As part of the BBS/CCT complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. Known to play a role, in vitro, in the folding of actin and tubulin.1) Mukherjee, K., et al. BMC Evol. Biol. 10, 64 (2010) : |
| Immunogen | This CCT8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 519-548 amino acids from the C-terminal region of human CCT8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CCT8 |
|---|---|
| Alternative Names | T-complex protein 1 subunit theta TCP-1-theta CCT-theta Renal carcinoma antigen NY-REN-15 CCT8 C21orf112 CCTQ KIAA0002 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Monkey |
Application
Flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.
Application
CCT8 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in K562 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
