Application
Western blot analysis of extract from Raji cells using CD22 Antibody.| Product Name | CD22 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SIGLEC2, SIGLEC-2, RAF, SIGLEC2, FLJ22814, MGC130020, |
| Product description | Mediates B-cell B-cell interactions. May be involved in the localization of B-cells in lymphoid tissues. Binds sialylated glycoproteins; one of which is CD45. Preferentially binds to alpha-2, 6-linked sialic acid. The sialic acid recognition site can be masked by cis interactions with sialic acids on the same cell surface. Upon ligand induced tyrosine phosphorylation in the immune response seems to be involved in regulation of B-cell antigen receptor signaling. Plays a role in positive regulation through interaction with Src family tyrosine kinases and may also act as an inhibitory receptor by recruiting cytoplasmic phosphatases via their SH2 domains that block signal transduction through dephosphorylation of signaling molecules.1) Doody G. M., Justement L. B., Delibrias C. C., Matthews R. J., Lin J., Thomas M. L., Fearon D. T. Science 269:242-244 (1995) Tuscano J. M., Engel P., Tedder T. F., Agarwal A., Kehrl J. H. Eur. J. Immunol. 26:1246-1252 (1996) Law C. L., Sidorenko S. P., Chandran K. A., Zhao Z., Shen S. H., Fischer E. H., Clark E. A. J. Exp. Med. 183:547-560 (1996) |
| Immunogen | CD22 antibody was raised against a peptide sequence around aa.839~843 (N-V-D-Y-V) derived from Human CD22. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD22 |
|---|---|
| Alternative Names | SIGLEC2 SIGLEC-2 RAF SIGLEC2 FLJ22814 MGC130020 |
| Molecular Weight(MW) | 140 kDa |
| Tissue Specificity | This antibody detects endogenous level of total CD22 protein. |
| Entrez Gene | 933 |
|---|---|
| Protein Accession | NP_001762.2 |
Application
Western blot analysis of extract from Raji cells using CD22 Antibody.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
