Application
Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 ζ (Phospho-Tyr142) Antibody. The lane on the left is treated with antigen-specific peptide.| Product Name | CD3 zeta (phospho Tyr142) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Probable role in assembly and expression of the TCR complex as well as signal transduction upon antigen triggering.1) Linda A. Trimble, Blood, Aug 2000; 96: 1021 - 1029. |
| Immunogen | CD3 zeta (phospho Tyr142) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 142 (G-L-Y (p)-Q-G) derived from Human CD3 ζ. |
| Modification | p-Tyr142 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD247 |
|---|---|
| Alternative Names | T3Z CD3-zeta TCRZ T-cell receptor T3 zeta chain |
| Molecular Weight(MW) | 20 kDa |
| Tissue Specificity | The antibody detects endogenous levels of CD3 ζ only when phosphorylated at tyrosine 142. |
| Entrez Gene | 919 |
|---|---|
| Protein Accession | NP_000725.1 |
Application
Western blot analysis of extracts from JK cells (Lane 2) and K562 cells (Lane 3), using CD3 ζ (Phospho-Tyr142) Antibody. The lane on the left is treated with antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
