Application
FCM analysis CD3 expressed in human peripheral blood cells.| Product Name | CD3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD3Z, T3Z, TCRZ, T-cell receptor T3 zeta chainT3Z, CD3H, CD3Q, CD3Z, CD3-ZETA |
| Product description | CD3 is a component of T cell surface receptor (TCR), it cantains 5 invariable chains: gamma- , delta-, epsilon-, and zeta/eta。Clevers et al. (1988) showed the TCR/CD3 complex consists of either a TCR alpha/beta or TCR gamma/delta heterodimer coexpressed at the cell surface with the invariant subunits，CD3 is responsible for the signal transduction of the TCR.1) Clevers,H.Annu.Rev.Immun.6:629-662,1988. |
| Immunogen | Ni-NTA purified recombinant human CD3 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG2a |
| Host Species | Mouse |
| Tested Applications | ELISAFACS |
| Flow Cytometry and ELISA. Dilution: Flow Cytometry :1:200 - 1:400 ELISA:1:10000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CD247 |
|---|---|
| Alternative Names | CD3Z T3Z TCRZ T-cell receptor T3 zeta chainT3Z CD3H CD3Q CD3Z CD3-ZETA |
Application
FCM analysis CD3 expressed in human peripheral blood cells.| Positive Control | 1) Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate |
|---|---|
| Application Notes | Flow Cytometry and ELISA. Dilution: Flow Cytometry :1:200 - 1:400 ELISA:1:10000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CD3 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
