Application
Western blot analysis of extract from hematopoietic stem cells using CD31 (PECAM1).| Product Name | CD31 (PECAM1) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD31, PECA1, GPIIA', PECAM-1, endoCAM, CD31/EndoCAM, MCH5, CD31, FLJ34100, FLJ58394, PECAM1 |
| Product description | Induces susceptibility to atherosclerosis By similarity. Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Prevents phagocyte ingestion of closely apposed viable cells by transmitting 'detachment' signals, and changes function on apoptosis, promoting tethering of dying cells to phagocytes (the encounter of a viable cell with a phagocyte via the homophilic interaction of PECAM1 on both cell surfaces leads to the viable cell's active repulsion from the phagocyte. During apoptosis, the inside-out signaling of PECAM1 is somehow disabled so that the apoptotic cell does not actively reject the phagocyte anymore. The lack of this repulsion signal together with the interaction of the eat-me signals and their respective receptors causes the attachment of the apoptotic cell to the phagocyte, thus triggering the process of engulfment). Isoform Delta15 is unable to protect against apoptosis. Modulates BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in human umbilical cord vein cells (HUVEC).1) Brown S., Heinisch I., Ross E., Shaw K., Buckley C. D., Savill J. Nature 418:200-203 (2002) Bergom C., Paddock C., Gao C., Holyst T., Cell Sci. 121:1235-1242 (2008) Dasgupta B., Dufour E., Mamdouh Z., Muller W. A. J. Immunol. 182:5041-5051 (2009) |
| Immunogen | CD31 (PECAM1) antibody was raised against a peptide sequence around aa.730~734 (R-T-E-G-S) derived from Human CD31 (PECAM1). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PECAM1 |
|---|---|
| Alternative Names | CD31 PECA1 GPIIA' PECAM-1 endoCAM CD31/EndoCAM MCH5 CD31 FLJ34100 FLJ58394 PECAM1 |
| Molecular Weight(MW) | 130 kDa |
| Tissue Specificity | This antibody detects endogenous level of total CD31 protein. |
| Entrez Gene | 5175 |
|---|---|
| Protein Accession | NP_000433.4 |
Application
Western blot analysis of extract from hematopoietic stem cells using CD31 (PECAM1).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM274436