Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: human lung lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis of CD31 antibody in HL60 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human lung carcinoma with CD31 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH1| Product Name | CD31 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Platelet endothelial cell adhesion molecule, PECAM-1, EndoCAM, GPIIA', PECA1, CD31, PECAM1 |
| Product description | CD31 is a cell adhesion molecule expressed on platelets and at endothelial cell intercellular junctions.1) Simmons D.L., Walker C.J. Exp. Med. 171:2147-2152(1990) |
| Immunogen | This CD31 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 690-718 amino acids from the C-terminal region of human CD31. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PECAM1 |
|---|---|
| Alternative Names | Platelet endothelial cell adhesion molecule PECAM-1 EndoCAM GPIIA' PECA1 CD31 PECAM1 |
| Molecular Weight(MW) | 83 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Jurkat whole cell lysate Lane 2: human lung lysate Lane 3: THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis of CD31 antibody in HL60 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human lung carcinoma with CD31 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Overlay histogram showing THP-1 cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OH1| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM274437