Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Product Name | CD320 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD320 antigen, 8D6 antigen, FDC-signaling molecule 8D6, FDC-SM-8D6, Transcobalamin receptor, TCblR, CD320, CD320, 8D6A |
| Product description | The CD320 antigen gene encodes the transcobalamin receptor (TCBLR). Cellular uptake of cobalamin (vitamin B12) is mediated by receptors expressed on the cell surface. Transcobalamin II (TCN2; MIM 613441), a plasma protein secreted by endothelial cells, binds the cobalamin absorbed in the distal ileum and carries 10 to 30% of total circulating cobalamin. CD320 encodes a transcobalamin receptor that binds TCN2-cobalamin at the plasma membrane and internalizes the complex by endocytosis (Quadros et al., 2009 [PubMed 18779389]).1) Jiang, W., et al. Gene 466 (1-2), 49-55 (2010) : |
| Immunogen | This CD320 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 153-182 amino acids from the C-terminal region of human CD320. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD320 |
|---|---|
| Alternative Names | CD320 antigen 8D6 antigen FDC-signaling molecule 8D6 FDC-SM-8D6 Transcobalamin receptor TCblR CD320 CD320 8D6A |
| Molecular Weight(MW) | 29 kDa |
Application
Western blot analysis in U251 cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
