Application
Flow cytometric analysis of jurkat cells using CD45 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Antibody is used in Western blot to detect CD45 in jurkat cell lysate.| Product Name | CD45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, L-CA, T200, CD45, PTPRC, CD45 |
| Product description | CD45 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. The CD45 gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling.1) Stanton, T., et al., Proc. Natl. Acad. Sci. U.S.A. 100(10):5997-6002 (2003). Vogel, A., et al., Genes Immun. 4(1):79-81 (2003). Rachmilewitz, J., et al., J. Biol. Chem. 278(16):14059-14065 (2003). McCann, F.E., et al., J. Immunol. 170(6):2862-2870 (2003). Fernandis, A.Z., et al., J. Biol. Chem. 278(11):9536-9543 (2003). |
| Immunogen | This CD45 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1245-1275 amino acids from the C-terminal region of human CD45. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PTPRC |
|---|---|
| Alternative Names | Receptor-type tyrosine-protein phosphatase C Leukocyte common antigen L-CA T200 CD45 PTPRC CD45 |
| Molecular Weight(MW) | 147 kDa |
Application
Flow cytometric analysis of jurkat cells using CD45 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human tonsil reacted with CD45 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Antibody is used in Western blot to detect CD45 in jurkat cell lysate.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
