| Product Name | CD45 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | LCA, LY5, B220, CD45, L-CA, T200, CD45R, GP180, PTPRC |
| Product description | The HI30 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. It is expressed on lymphocytes, granulocytes, monocytes, thymocytes, and eosinophils, but not on mature erythrocytes, platelets, mature erythroid cells of bone marrow, and non-hematopoietic tissues. CD45 is essential for T cell activation and the tyrosine phosphatase activity of its intracellular region is integral for signal transduction.1) Knapp W(1989) Leucocyte typing IV: white cell differentiation antigens. Oxford University Press, 1989. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1, k |
| Host Species | Mouse |
| Tested Applications | FACSFunctionalStudiesICCIHCIPWB |
| FACS:1:200~1:500 ICC:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PTPRC |
|---|---|
| Alternative Names | LCA LY5 B220 CD45 L-CA T200 CD45R GP180 PTPRC |
| Tissue Specificity | The HI30 monoclonal antibody specifically reacts with the 180 kDa, 190 kDa, 205 kDa, and 220 kDa isoforms of the human leukocyte common antigen (LCA) CD45. |
| Entrez Gene | 5788 |
|---|
| Application Notes | FACS:1:200~1:500 ICC:1:200~1:500 IHC:1:50~1:200 IP:1:100~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The product should be stored undiluted at 4˚C and should be protected from prolonged exposure to light. Do not freeze. |
| Storage Buffer | Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2. |
Data sheet for OM274656