Application
Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD8.| Product Name | CD8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | MALCD8, MAL, p32, Leu2 |
| Product description | CD8 T cell surface antigen is heterodimer of an alpha and a beta chain linked by two disulfide bonds .It belongs type I membrane protein. Selectively expressing of CD8 on a subset of T cells leads to CD8 T cell development. Through identifying cytotoxic/suppressor T-cells that interact with MHC class I bearing targets, CD8 is thought to play a role in the process of T-cell mediated killing. Veillette et al (1988) found the CD8 is associated with the internal membrane tyrosine-protein kinase p56lck.1) Veillette, A. et al. 1988. Cell 55:301. |
| Immunogen | Ni-NTA purified recombinant human CD8 expressed in E. Coli strain BL21 (DE3). |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD8A |
|---|---|
| Alternative Names | MALCD8 MAL p32 Leu2 |
Application
Flow cytometric analysis of peripheral blood T cells using monoclonal antibody to human CD8.| Positive Control | 1) Cat. No. 1371 - Human peripheral blood leukocyte (PBL) Lysate |
|---|---|
| Application Notes | Western Blot:1:500 - 1,000 ELISA:Determining optimal working dilutions by titration test.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CD8 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide |
