Application
Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 μg/mL.Application
Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 μg/mL.Application
Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking petide.| Product Name | CD81 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD81 Antibody: S5.7, CVID6, TAPA1, TSPAN28, CD81 antigen, 26 kDa cell surface protein TAPA-1, Tspan-28 |
| Product description | CD81 Antibody: CD81 is a member of the transmembrane 4 superfamily, also known as the tetraspanin family, a group of cell-surface proteins that are characterized by the presence of four hydrophobic domains and mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. CD81 is a cell surface glycoprotein that associates with integrins and appears to promote muscle cell fusion and support myotube maintenance. Along with SCARB1, LDL-R, and CLDN1, CD81 has been reported to be an entry factor for the Hepatitis C virus. Finally, recent evidence indicates that the CD81 gene is localized in a tumor-suppressor gene region and is thus a candidate gene for malignancies.1) Oren R, Takahashi S, Doss C, et al. TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol. Cell Biol. 1990; 10:4007-15. |
| Immunogen | CD81 antibody was raised against a 20 amino acid synthetic peptide near the amino terminus of human CD81. The immunogen is located within amino acids 30 - 80 of CD81. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CD81 |
|---|---|
| Alternative Names | CD81 Antibody: S5.7 CVID6 TAPA1 TSPAN28 CD81 antigen 26 kDa cell surface protein TAPA-1 Tspan-28 |
| Molecular Weight(MW) | Predicted: 26 kDa Observed: 25 kDa |
| Sequence Similarities | 3) Cat. No. 17-003 - A549 Cell Slide |
Application
Immunohistochemistry of CD81 in human lung tissue with CD81 antibody at 5 μg/mL.
Application
Immunofluorescence of CD81 in A549 cells with CD81 antibody at 20 μg/mL.
Application
Western blot analysis of CD81 in human lung tissue lysate with CD81 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking petide.| Positive Control | 1) Cat. No. 1283 - RAW 264.7 Cell Lysate |
|---|---|
| Application Notes | CD81 antibody can be used for detection of CD81 by Western blot at 2.5 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CD81 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CD81 Antibody is supplied in PBS containing 0.02% sodium azide. |
