Application
Western blot analysis of lysed extracts from 3T3 cells untreated (Lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149).| Product Name | Cdc25B (phospho Ser149) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | AI604853, CDC25B |
| Product description | Cdc25B is a member of the CDC25 family of phosphatases. CDC25B activates the cyclin dependent kinase CDC2 by removing two phosphate groups and it is required for entry into mitosis. CDC25B shuttles between the nucleus and the cytoplasm due to nuclear localization and nuclear export signals. The protein is nuclear in the M and G1 phases of the cell cycle and moves to the cytoplasm during S and G2. CDC25B has oncogenic properties, although its role in tumor formation has not been determined. Multiple transcript variants for this gene exist.1) Li G, et al. (2010) Retrovirology. 7:59. Wang Z, et al. (2010) BMC Cancer. 10:233. Lucci MA, et al. (2010) Cell Oncol. 32 (5-6): 361-72. |
| Immunogen | Cdc25B (Phospho-Ser149) antibody was raised against a peptide sequence around phosphorylation site of Serine 149 (F-R-S (p) -L-P) derived from Mouse Cdc25B. |
| Modification | p-Ser149 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | Cdc25b |
|---|---|
| Alternative Names | AI604853 CDC25B |
| Molecular Weight(MW) | 62 kDa |
| Tissue Specificity | This antibody detects endogenous level of Cdc25B protein only when phosphorylated at serine 149. |
| Entrez Gene | 12531 |
|---|---|
| Protein Accession | NP_001104545.1 |
Application
Western blot analysis of lysed extracts from 3T3 cells untreated (Lane 1) or treated with Hydroxyurea (lane 2) using Cdc25B (Phospho-Ser149).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
