Application
Flow cytometric analysis of NCI-H292 cells using CDH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CDH3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CDH3 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cadherin-3, Placental cadherin, P-cadherin, CDH3, CDHP |
| Product description | CDH3 is a classical cadherin from the cadherin superfamily. It is a calcium-dependent cell-cell adhesion glycoprotein comprised of five extracellular cadherin repeats, a transmembrane region and a highly conserved cytoplasmic tail. Aberrant expression of this protein is observed in cervical adenocarcinomas. Mutations in the gene encoding CDH3 have been associated with congential hypotrichosis with juvenile macular dystrophy.1) Indelman,M., Clin. Exp. Dermatol. 32 (2), 191-196 (2007) |
| Immunogen | This CDH3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 114-142 amino acids from the N-terminal region of human CDH3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDH3 |
|---|---|
| Alternative Names | Cadherin-3 Placental cadherin P-cadherin CDH3 CDHP |
| Molecular Weight(MW) | 91 kDa |
Application
Flow cytometric analysis of NCI-H292 cells using CDH3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with CDH3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western Blot at 1:1000 dilution + A431 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
