CDK5 is probably involved in the control of the cell cycle. It interacts with D1 and D3-type G1 cyclins. The protein can phosphorylate histone H1, tau, MAP2 and NF-H and NF-M. And it is also thought to interacts with p35 which activates the kinase.1) Liu, R., (2008) Proc. Natl. Acad. Sci. U.S.A. 105 (21), 7570-7575.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CDK5 antibody can be used for detection of CDK5 by ELISA at 1:62500. CDK5 antibody can be used for detection of CDK5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
CDK5 antibody can be used for detection of CDK5 by ELISA at 1:62500. CDK5 antibody can be used for detection of CDK5 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CDK5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.