Application
Western blot analysis in A2058 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | CDK5R1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cyclin-dependent kinase 5 activator 1, CDK5 activator 1, Cyclin-dependent kinase 5 regulatory subunit 1, TPKII regulatory subunit, Cyclin-dependent kinase 5 activator 1, p35, p35, Cyclin-dependent kinase 5 activator 1, p25, p25, Tau protein kinase II 23 kD |
| Product description | p35 is a neuron-specific activator of cyclin-dependent kinase 5 (CDK5); the activation of CDK5 is required for proper development of the central nervous system. The p35 form of this protein is proteolytically cleaved by calpain, generating a p25 form. The cleavage of p35 into p25 results in relocalization of the protein from the cell periphery to nuclear and perinuclear regions. P25 deregulates CDK5 activity by prolonging its activation and changing its cellular location. The p25 form accumulates in the brain neurons of patients with Alzheimer's disease. This accumulation correlates with an increase in CDK5 kinase activity, and may lead to aberrantly phosphorylated forms of the microtubule-associated protein tau, which contributes to Alzheimer's disease.1) Lin,S., FEBS Lett. 582 (8), 1197-1202 (2008) |
| Immunogen | This CDK5R1(p35) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 272-301 amino acids from the C-terminal region of human CDK5R1(p35). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK5R1 |
|---|---|
| Alternative Names | Cyclin-dependent kinase 5 activator 1 CDK5 activator 1 Cyclin-dependent kinase 5 regulatory subunit 1 TPKII regulatory subunit Cyclin-dependent kinase 5 activator 1 p35 p35 Cyclin-dependent kinase 5 activator 1 p25 p25 Tau protein kinase II 23 kD |
| Molecular Weight(MW) | 34 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in A2058 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with CDK5R1(p35) Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
