Application
Top Image: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr24).Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24).| Product Name | CDK6 (phospho Tyr24) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PLSTIRE, CREB2, CREBP1, Serine/threonine-protein kinase PLSTIRE |
| Product description | Probably involved in the control of the cell cycle. Interacts with D-type G1 cyclins.1) Flores-Rozas, H. et al. (1994) Proc. Natl. Acad. Sci. USA 91, 8655-8659. Pestell, R. G. et al. (1999) Endocr. Rev. 20, 501-534. Lukas, J. et al. (1996) Mol. Cell. Biol. 16, 6917-1625. |
| Immunogen | CDK6 (phospho-Tyr24) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 24 (G-A-Y (p) -G-K) derived from Human CDK6. |
| Modification | p-Tyr24 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDK6 |
|---|---|
| Alternative Names | PLSTIRE CREB2 CREBP1 Serine/threonine-protein kinase PLSTIRE |
| Molecular Weight(MW) | 36 kDa |
| Tissue Specificity | This antibody detects endogenous level of CDK6 only when phosphorylated at tyrosine 24. |
| Entrez Gene | 1021 |
|---|---|
| Protein Accession | NP_001138778.1 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using CDK6 (Phospho-Tyr24). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using CDK6 (phospho-Tyr24).
Application
Western blot analysis of lysed extracts from 293 cells untreated (Lane 1) or treated with starvation (lane 2) using CDK6 (phospho-Tyr24).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
