Application
Antibody used in WB on Human 293T at 2.5 ug/ml.| Product Name | CDK9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CDK9, RP11-228B15.5, C-2k, CDC2L4, PITALRE, TAK, CTK1 |
| Product description | CDK9 is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.The protein encoded by this gene is a member of the cyclin-dependent protein kinase (CDK) family. CDK family members are highly similar to the gene products of S. cerevisiae cdc28, and S. pombe cdc2, and known as important cell cycle regulators. This kinase was found to be a component of the multiprotein complex TAK/P-TEFb, which is an elongation factor for RNA polymerase II-directed transcription and functions by phosphorylating the C-terminal domain of the largest subunit of RNA polymerase II. This protein forms a complex with and is regulated by its regulatory subunit cyclin T or cyclin K. HIV-1 Tat protein was found to interact with this protein and cyclin T, which suggested a possible involvement of this protein in AIDS.1) Li, Q., (2005) J. Biol. Chem. 280 (31), 28819-28826. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK9. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| CDK9 antibody can be used for detection of CDK9 by ELISA at 1:312500. CDK9 antibody can be used for detection of CDK9 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CDK9 |
|---|---|
| Alternative Names | CDK9 RP11-228B15.5 C-2k CDC2L4 PITALRE TAK CTK1 |
| Molecular Weight(MW) | 43 kDa |
Application
Antibody used in WB on Human 293T at 2.5 ug/ml.| Positive Control | 1) Tranfected 293T Cell Lysate |
|---|---|
| Application Notes | CDK9 antibody can be used for detection of CDK9 by ELISA at 1:312500. CDK9 antibody can be used for detection of CDK9 by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CDK9 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
