Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.Application
Antibody used in IHC on Human Skin.| Product Name | CENPA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA is a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.Centromeres are the differentiated chromosomal domains that specify the mitotic behavior of chromosomes. CENPA encodes a centromere protein which contains a histone H3 related histone fold domain that is required for targeting to the centromere. CENPA is proposed to be a component of a modified nucleosome or nucleosome-like structure in which it replaces 1 or both copies of conventional histone H3 in the (H3-H4)2 tetrameric core of the nucleosome particle.1) Black, B.E., (2004) Nature 430 (6999), 578-582. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CENPA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| CENPA antibody can be used for detection of CENPA by ELISA at 1:1562500. CENPA antibody can be used for detection of CENPA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | CENPA |
|---|---|
| Alternative Names | CENPA CenH3 CENP-A |
| Molecular Weight(MW) | 16 kDa, 13 kDa |
Application
Antibody used in WB on Human Jurkat cells at 1.25 ug/ml.
Application
Antibody used in IHC on Human Skin.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | CENPA antibody can be used for detection of CENPA by ELISA at 1:1562500. CENPA antibody can be used for detection of CENPA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store CENPA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
