Application
Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.| Product Name | CENPO Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPO Antibody: CENP-O, MCM21R, ICEN-36, ICEN36, Centromere protein O, Interphase centromere complex protein 36, CENP-O |
| Product description | CENPO Antibody: Accurate chromosome segregation during mitosis requires a kinetochore to assemble correctly at the centromere of each chromatid and form a dynamic interface with the microtubules of the mitotic spindle. The kinetochore assembly includes the multisubunit CENP-H/I complex which can be divided into three functional classes based on phenotype analysis. One of these classes is referred to as the CENP-O class of proteins of which CENPO is the exemplar. These proteins form a stable complex with each other and are required for proper kinetochore function and for recovery from mitotic spindle damage. CENPO has also been shown to be an auto-antigen in a small population of anti-centromere antibodies- (ACA-) positive patients with sclerodoma.1) Fukagawa T. Assembly of kinetochore in vertebrate cells. Exp. Cell Res.2004; 296:21-7. |
| Immunogen | CENPO antibody was raised against a 16 amino acid synthetic peptide from near the center of human CENPO. The immunogen is located within amino acids 170 - 220 of CENPO. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CENPO |
|---|---|
| Alternative Names | CENPO Antibody: CENP-O MCM21R ICEN-36 ICEN36 Centromere protein O Interphase centromere complex protein 36 CENP-O |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (100%) |
Application
Western blot analysis of CENPO in mouse kidney tissue lysate with CENPO antibody at (A) 1 and (B) 2 μg/mL.| Positive Control | 1) Cat. No. 1405 - Mouse Kidney Tissue Lysate |
|---|---|
| Application Notes | CENPO antibody can be used for detection of CENPO by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CENPO antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CENPO Antibody is supplied in PBS containing 0.02% sodium azide. |
