Application
Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.| Product Name | CENPW Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CENPW Antibody: CUG2, CENP-W, C6orf173, CUG2, Centromere protein W, Cancer-up-regulated gene 2 protein |
| Product description | CENPW Antibody: CENPW was initially identified as a gene that was upregulated in multiple cancers, and whose overexpression in mouse fibroblast cells gave rise to distinct cancer-specific phenotypes. It was later found to be a nuclear protein that associated with CENPT, a component of CENPA nucleosome complex in the centromere, and is required for proper kinetochore function. CENPW also specifically interacts with the nucleolar phosphoprotein nucleophosmin, also known as B23 It has been suggested that nucleophosmin functions in the assembly of the kinetochore by interacting with CENPW during interphase. Overexpression of CENPW in the SKOV-3 human ovarian cancer cell line as well as in the zebrafish embryo led to apoptosis, suggesting that high levels of CENPW induces apoptotic cell death.1) Lee S, Gang J, Jeon SB, et al. molecular cloning and functional analysis of a novel oncogene, cancer-upregulated gene 2 (CUG2). Biochem. Biophys. Res. Commun. 2007; 360:633-9. |
| Immunogen | CENPW antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human CENPW. The immunogen is located within the first 50 amino acids of CENPW. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CENPW |
|---|---|
| Alternative Names | CENPW Antibody: CUG2 CENP-W C6orf173 CUG2 Centromere protein W Cancer-up-regulated gene 2 protein |
| Molecular Weight(MW) | kDa |
| Tissue Specificity | At least three isoforms of CENPW are known to exist; this antibody will detect all three isoforms. |
| Entrez Gene | 387103 |
|---|---|
| Protein Accession | NP_001012525 |
Application
Western blot analysis of CENPW in HeLa cell lysate lysate with CENPW antibody at (A) 0.5 and (B) 1 μg/mL.| Positive Control | 1) Cat. No. 1201 - HeLa Cell Lysate |
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| Application Notes | CENPW antibody can be used for detection of CENPW by Western blot at 0.5 - 1 μg/mL.: |
| Form | Liquid |
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| Storage Instructions | CENPW antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CENPW Antibody is supplied in PBS containing 0.02% sodium azide. |
