Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used foApplication
Western blot analysis of CER1 Antibody in NCI-H460 cell line lysates (35ug/lane)| Product Name | CER1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cerberus, Cerberus-related protein, DAN domain family member 4, CER1, DAND4 |
| Product description | CER1 is a cytokine member of the cysteine knot superfamily, characterized by nine conserved cysteines and a cysteine knot region. The cerberus-related cytokines, together with Dan and DRM/Gremlin, represent a group of bone morphogenetic protein (BMP) antagonists that can bind directly to BMPs and inhibit their activity.1) Tang,P.L., et.al., J. Bone Miner. Res. 24 (6), 1013-1021 (2009) |
| Immunogen | This CER1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 56-85 amino acids from the N-terminal region of human CER1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | CER1 |
|---|---|
| Alternative Names | Cerberus Cerberus-related protein DAN domain family member 4 CER1 DAND4 |
| Molecular Weight(MW) | 30 kDa |
Application
(LEFT)Formalin-fixed and paraffin-embedded human lung carcinoma with CER1 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. (RIGHT)Flow cytometric analysis of NCI-H292 cells using 0.025mg/ml of CER1 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used fo
Application
Western blot analysis of CER1 Antibody in NCI-H460 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
