Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: LNCaP whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Product Name | CERS4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Ceramide synthase 4, CerS4, LAG1 longevity assurance homolog 4, CERS4, LASS4 |
| Product description | May be either a bona fide (dihydro)ceramide synthase or a modulator of its activity. When overexpressed in cells is involved in the production of sphingolipids containing different fatty acid donors (N-linked stearoyl- (C18) or arachidoyl- (C20) ceramides) in a fumonisin B1-independent manner (By similarity).1) Ota T.,et al.Nat. Genet. 36:40-45(2004). |
| Immunogen | This CERS4 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 21-51 amino acids from human CERS4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSWB |
| For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CERS4 |
|---|---|
| Alternative Names | Ceramide synthase 4 CerS4 LAG1 longevity assurance homolog 4 CERS4 LASS4 |
| Molecular Weight(MW) | 46 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: HT-1080 whole cell lysate Lane 3: Jurkat whole cell lysate Lane 4: LNCaP whole cell lysate Lane 5: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Overlay histogram showing Hela cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37ºC. The secondary antibody used was Goat-Anti-Rabbit Ig| Application Notes | For FACS starting dilution is: 1:25 For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
