Application
CFL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of lysate from NCI-H1299 cell line, using CFL2 Antibody at 1:1000 at each lane.| Product Name | CFL2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cofilin-2, Cofilin, muscle isoform, CFL2 |
| Product description | This gene encodes an intracellular protein that is involved in the regulation of actin-filament dynamics. This protein is a major component of intranuclear and cytoplasmic actin rods. It can bind G- and F-actin in a 1:1 ratio of cofilin to actin, and it reversibly controls actin polymerization and depolymerization in a pH-dependent manner. Mutations in this gene cause nemaline myopathy type 7, a form of congenital myopathy. Alternative splicing results in multiple transcript variants.1) Vogel, S., et al. J. Biol. Chem. 285(44):33756-33763(2010) Borensztajn, K., et al. Thromb. Res. 125 (6), E323-E328 (2010)Papalouka, V., et al. Mol. Cell. Biol. 29(22):6046-6058(2009) Wu, Y., et al. Retrovirology 5, 95 (2008)Agrawal, P.B., et al. Am. J. Hum. Genet. 80(1):162-167(2007) |
| Immunogen | This CFL2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 25-54 amino acids from the N-terminal region of human CFL2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CFL2 |
|---|---|
| Alternative Names | Cofilin-2 Cofilin muscle isoform CFL2 |
| Molecular Weight(MW) | 19 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
CFL2 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human rectum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of lysate from NCI-H1299 cell line, using CFL2 Antibody at 1:1000 at each lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
