Application
Western blot analysis of CHCHD6 in SK-N-SH cell lysate with CHCHD6 antibody at 1 μg/mL.| Product Name | CHCHD6 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHCHD6 Antibody: CHCM1, PPP1R23, CHCM1, Coiled-coil-helix-coiled-coil-helix domain-containing protein 6, mitochondrial, Coiled coil helix cristae morphology protein 1 |
| Product description | CHCHD6 Antibody: CHCHD6 is a member of a family of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain and has been observed in a complex with the mitochondrial proteins mitofilin, SAM50, metaxins 1 and 2, and CHCHD3. CHCHD6 knockdown causes severe defects in mitochondrial cristae morphology as well as reductions in cell growth, ATP production and oxygen consumption. This decrease of CHCHD6 also led to a reduction in mitofilin protein levels, while a knockdown of mitofilin resulted in a reduced level of CHCHD6, suggesting coordinate regulation between these two proteins. CHCHD6 knockdown in human cancer cells enhances their sensitivity to genotoxic anticancer drugs, suggesting that CHCHD6 may be a potential therapeutic anti-tumor target.1) Xie J, Marusich MF, Souda P, et al. The mitochondrial inner membrane protein Mitofilin exists as a complex with SAM50, metaxins 1 and 2, coiled-coil-helix coiled-coil-helix domain-containing protein 3 and 6 and DnaJC11. FEBS Lett. 2007; 581:3545-9. |
| Immunogen | Rabbit polyclonal CHCHD6 antibody was raised against a 17 amino acid peptide near the amino terminus of human CHCHD6. The immunogen is located within amino acids 20 - 70 of CHCHD6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CHCHD6 antibody can be used for detection of CHCHD6 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHCHD6 |
|---|---|
| Alternative Names | CHCHD6 Antibody: CHCM1 PPP1R23 CHCM1 Coiled-coil-helix-coiled-coil-helix domain-containing protein 6 mitochondrial Coiled coil helix cristae morphology protein 1 |
| Molecular Weight(MW) | Predicted: 26 kDa |
| Tissue Specificity | CHCHD6 is predicted to not cross-react to other members of the CHCHD family. |
Application
Western blot analysis of CHCHD6 in SK-N-SH cell lysate with CHCHD6 antibody at 1 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | CHCHD6 antibody can be used for detection of CHCHD6 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CHCHD6 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CHCHD6 Antibody is supplied in PBS containing 0.02% sodium azide. |
