Application
Western blot analysis of CHCHD7 in rat liver tissue lysate with CHCHD7 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Product Name | CHCHD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHCHD7 Antibody: COX23, Coiled-coil-helix-coiled-coil-helix domain-containing protein 7 |
| Product description | CHCHD7 Antibody: CHCHD7 is a newly identified member of a multifamily of proteins containing a conserved (coiled coil 1)-(helix 1)-(coiled coil 2)-(helix 2) domain. Northern blot analysis revealed that the gene is ubiquitously expressed. Its biological function is unknown and the gene has hitherto not been associated with neoplasia. CHCHD7 and PLAG1 are located head-to-head about 500 bp apart in 8q12. A chromosomal aberration involving CHCHD7 is found in salivary gland pleiomorphic adenomas, the most common benign epithelial tumors of the salivary gland.1) Asp J, Persson F, Kost-Alimova M, et al. CHCHD7-PLAG1 and TCEA1-PLAG1 gene fusions resulting from cryptic, intrachromosomal 8q rearrangements in pleomorphic salivary gland adenomas. Genes Chromosomes Cancer 2006; 45:820-8. |
| Immunogen | Rabbit polyclonal CHCHD7 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human CHCHD7. The immunogen is located within the last 50 amino acids of CHCHD7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| CHCHD7 antibody can be used for detection of CHCHD7 by Western blot at 1 - 2 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHCHD7 |
|---|---|
| Alternative Names | CHCHD7 Antibody: COX23 Coiled-coil-helix-coiled-coil-helix domain-containing protein 7 |
| Molecular Weight(MW) | Predicted: 11 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine: (71%) |
| Tissue Specificity | Three alternatively spliced transcript variants have been observed. CHCHD7 is predicted to not cross-react to other members of the CHCHD family. |
Application
Western blot analysis of CHCHD7 in rat liver tissue lysate with CHCHD7 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.| Positive Control | 1) Cat. No. 1464 - Rat Liver Tissue Lysate |
|---|---|
| Application Notes | CHCHD7 antibody can be used for detection of CHCHD7 by Western blot at 1 - 2 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CHCHD7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | CHCHD7 Antibody is supplied in PBS containing 0.02% sodium azide. |
