Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | CHD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Chromodomain-helicase-DNA-binding protein 5, CHD-5, 3.6.4.12, ATP-dependent helicase CHD5, CHD5 {ECO:0000312|EMBL:AAL98962.1}, KIAA0444 |
| Product description | Chromatin-remodeling protein that binds DNA through histones and regulates gene transcription. May specifically recognize and bind trimethylated 'Lys-27' (H3K27me3) and non- methylated 'Lys-4' of histone H3. Plays a role in the development of the nervous system by activating the expression of genes promoting neuron terminal differentiation. In parallel, it may also positively regulate the trimethylation of histone H3 at 'Lys- 27' thereby specifically repressing genes that promote the differentiation into non-neuronal cell lineages. Tumor suppressor, it regulates the expression of genes involved in cell proliferation and differentiation. Downstream activated genes may include CDKN2A that positively regulates the p53/TP53 pathway, which in turn, prevents cell proliferation. In spermatogenesis, it probably regulates histone hyperacetylation and the replacement of histones by transition proteins in chromatin, a crucial step in the condensation of spermatid chromatin and the production of functional spermatozoa.1) Thompson P.M.,et al.Oncogene 22:1002-1011(2003). |
| Immunogen | This CHD5 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 64-98 amino acids from human CHD5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHD5 |
|---|---|
| Alternative Names | Chromodomain-helicase-DNA-binding protein 5 CHD-5 3.6.4.12 ATP-dependent helicase CHD5 CHD5 {ECO:0000312|EMBL:AAL98962.1} KIAA0444 |
| Molecular Weight(MW) | 223 kDa |
Application
Western Blot at 1:2000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
