Application
Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.Application
Immunofluorescence of CHD7 in mouse brain tissue with CHD7 antibody at 20 μg/mL.Application
Immunohistochemistry of CHD7 in mouse brain tissue with CHD7 antibody at 5 μg/mL.| Product Name | CHD7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CHD7 Antibody: CRG, HH5, IS3, KAL5, KIAA1416, Chromodomain-helicase-DNA-binding protein 7, ATP-dependent helicase CHD7, CHD-7 |
| Product description | CHD7 Antibody: CHD proteins belong to a superfamily of proteins of ATP-dependent chromatin remodeling enzymes that have a unique combination of functional domains, including two N-terminal chromodomains, a SNF2-like ATPase/helicase domain and a DNA-binding domain. These proteins are thought to play a role in early embryonic development by affecting chromatin structure and gene expression. Mutations in one member of this family, CHD7, result in CHARGE syndrome. It colocalizes with embryonic stem (ES) cell master regulators OCT4/POU5F1, SOX2, and NANOG and is thought to modulate ES-specific gene transcription. Together with SOX2, CHD7 has been suggested to also regulate several human disease genes.1) Woodage T, Basrai MA, Baxevanis AD, et al. Characterization of the CHD family of proteins. Proc. Natl. Acad. Sci. USA 1997; 94:11472-7. |
| Immunogen | CHD7 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human CHD7. The immunogen is located within amino acids 670 - 720 of CHD7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHD7 |
|---|---|
| Alternative Names | CHD7 Antibody: CRG HH5 IS3 KAL5 KIAA1416 Chromodomain-helicase-DNA-binding protein 7 ATP-dependent helicase CHD7 CHD-7 |
| Tissue Specificity | Multiple isoforms of CHD7 are known to exist. |
Application
Western blot analysis of CHD7 in SK-N-SH cell lysate with CHD7 antibody at (A) 1 and (B) 2 μg/mL.
Application
Immunofluorescence of CHD7 in mouse brain tissue with CHD7 antibody at 20 μg/mL.
Application
Immunohistochemistry of CHD7 in mouse brain tissue with CHD7 antibody at 5 μg/mL.| Positive Control | 1) Cat. No. 1220 - SK-N-SH Cell Lysate |
|---|---|
| Application Notes | CHD7 antibody can be used for detection of CHD7 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | CHD7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | CHD7 Antibody is supplied in PBS containing 0.02% sodium azide. |
