Application
Western blot analysis using CHK1 monoclonal antibody against NIH / 3T3 cell lysate.| Product Name | CHK1 Antibody [2G11D5] |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Active recombinant full length human CHK1 containing an Nterminal GST tag. This protein has an apparent molecular weight of ~82 kDa. CHK1 appears to function downstream of several of the known fission yeast checkpoint gene products, including that encoded by rad3+, a gene with sequence similarity to the ATM gene mutated in patients with ataxia telangiectasia.1) EMBO J. 1998. 16: 545-554. |
| Immunogen | Ni-NTA purified recombinant protein CHK1 expressed in E. Coli strain M15. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG1 |
| Host Species | Mouse |
| Tested Applications | ELISAWB |
| Western Blot:Dilution 1:2,00 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHEK1 |
|---|---|
| Alternative Names | CHK1 |
| Entrez Gene | 1111 |
|---|---|
| Protein Accession | NP_001107593.1 |
Application
Western blot analysis using CHK1 monoclonal antibody against NIH / 3T3 cell lysate.| Application Notes | Western Blot:Dilution 1:2,00 - 1:1,000 ELISA:Propose dilution 1:10,000. Determining optimal working dilutions by titration test. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | CHK1 monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Purified antibody in PBS containing 0.03% sodium azide. |
