Application
CHRNA10 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis in NCI-H292,MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).| Product Name | CHRNA10 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuronal acetylcholine receptor subunit alpha-10, Nicotinic acetylcholine receptor subunit alpha-10, NACHR alpha-10, CHRNA10, NACHRA10 |
| Product description | CHRNA10 is an ionotropic receptor with a probable role in the modulation of auditory stimuli. Agonist binding may induce an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is permeable to a range of divalent cations including calcium, the influx of which may activate a potassium current which hyperpolarizes the cell membrane. In the ear, this may lead to a reduction in basilar membrane motion, altering the activity of auditory nerve fibers and reducing the range of dynamic hearing. This may protect against acoustic trauma.1) Saccone, N.L., et al. Genes Brain Behav. 9(7):741-750(2010) |
| Immunogen | This CHRNA10 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 179-206 amino acids from the Central region of human CHRNA10. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CHRNA10 |
|---|---|
| Alternative Names | Neuronal acetylcholine receptor subunit alpha-10 Nicotinic acetylcholine receptor subunit alpha-10 NACHR alpha-10 CHRNA10 NACHRA10 |
| Molecular Weight(MW) | 50 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Rat |
Application
CHRNA10 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human tonsil tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis in NCI-H292,MDA-MB453,ZR-75-1 cell line lysates (35ug/lane).| Application Notes | For IHC-P starting dilution is: 1:10~50 For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
