After binding acetylcholine, the ACHR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane.1) Beeson D.M.W, et al., (1993) Eur. J. Biochem. 215:229-238..
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CHRNE.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CHRNE antibody can be used for detection of CHRNE by ELISA at 1:375000. CHRNE antibody can be used for detection of CHRNE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
CHRNE antibody can be used for detection of CHRNE by ELISA at 1:375000. CHRNE antibody can be used for detection of CHRNE by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CHRNE antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt