CIAO1 belongs to the WD repeat CIA1 family. It seems to specifically modulate the transactivation activity of WT1.1) Ewing, R.M., Mol. Syst. Biol. 3, 89 (2007).
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CIAO1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CIAO1 antibody can be used for detection of CIAO1 by ELISA at 1:62500. CIAO1 antibody can be used for detection of CIAO1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human 721_B at 0.2-1 ug/ml.
Positive Control
1) 721_B Cell Lysate
Application Notes
CIAO1 antibody can be used for detection of CIAO1 by ELISA at 1:62500. CIAO1 antibody can be used for detection of CIAO1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CIAO1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.