Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).Application
CIAO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | CIAO1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Probable cytosolic iron-sulfur protein assembly protein CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037}, WD repeat-containing protein 39 {ECO:0000255|HAMAP-Rule:MF_03037}, CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037}, WDR39 |
| Product description | CIAO1 is an essential component of the cytosolic iron-sulfur (Fe/S) protein assembly machinery. Required for the maturation of extramitochondrial Fe/S proteins. Seems to specifically modulate the transactivation activity of WT1.1) Srinivasan, V., et al. Structure 15(10):1246-1257(2007) |
| Immunogen | This CIAO1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 155-183 amino acids from the Central region of human CIAO1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CIAO1 |
|---|---|
| Alternative Names | Probable cytosolic iron-sulfur protein assembly protein CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037} WD repeat-containing protein 39 {ECO:0000255|HAMAP-Rule:MF_03037} CIAO1 {ECO:0000255|HAMAP-Rule:MF_03037} WDR39 |
| Molecular Weight(MW) | 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis in HepG2 cell line lysates (35ug/lane).
Application
CIAO1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
