CIR may modulate splice site selection during alternative splicing of pre-mRNAs. CIR regulates transcription and acts as corepressor for RBPSUH by recruiting RBPSUH to the Sin3-histone deacetylase complex (HDAC). CIR is also required for RBPSUH-mediated repression of transcription.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CIR.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
CIR antibody can be used for detection of CIR by ELISA at 1:62500. CIR antibody can be used for detection of CIR by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
CIR antibody can be used for detection of CIR by ELISA at 1:62500. CIR antibody can be used for detection of CIR by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store CIR antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.