Application
CIRH1A Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of CIRH1A Antibody in Neuro-2a cell line lysates (35ug/lane)| Product Name | CIRH1A Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cirhin, CIRH1A, KIAA1988, UTP4 |
| Product description | CIRH1A encodes a WD40-repeat-containing protein that is localized to the nucleolus. Mutation of this gene causes North American Indian childhood cirrhosis, a severe intrahepatic cholestasis that results in transient neonatal jaundice, and progresses to periportal fibrosis and cirrhosis in childhood and adolescence.1) Yu, B., et al. Exp. Cell Res. 315(18):3086-3098(2009) Carlton, V.E., et al. Ann. Med. 36(8):606-617(2004) |
| Immunogen | This CIRH1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 116-143 amino acids from the N-terminal region of human CIRH1A. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CIRH1A |
|---|---|
| Alternative Names | Cirhin CIRH1A KIAA1988 UTP4 |
| Molecular Weight(MW) | 77 kDa |
Application
CIRH1A Antibody IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of CIRH1A Antibody in Neuro-2a cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
