Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1alpha (Phospho-Tyr294) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | CK-1alpha (phospho Tyr294) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KC1A, CKI-alpha, CSNK1A1, alpha isoform, Casein kinase I |
| Product description | Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. It can phosphorylate a large number of proteins. Participates in Wnt signaling. Phosphorylates CTNNB1 at 'Ser-45'. May play a role in segregating chromosomes during mitosis.1) Tapia C., FEBS Lett. 349:307-312(1994). |
| Immunogen | CK-1alpha (phospho Tyr294) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 294 (Y-D-Y (p)-T-F) derived from Human CK-1alpha. |
| Modification | p-Tyr294 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | MouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CSNK1A1, CSNK1A1L |
|---|---|
| Alternative Names | KC1A CKI-alpha CSNK1A1 alpha isoform Casein kinase I |
| Molecular Weight(MW) | 37 kDa |
| Tissue Specificity | The antibody detects endogenous levels of CK-1alpha only when phosphorylated at tyrosine 294. |
| Entrez Gene | 1452/122011 |
|---|---|
| Protein Accession | NP_001883.4 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using CK-1alpha (Phospho-Tyr294) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
